Journal: Scientific Reports

Article Title: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei

doi: 10.1038/s41598-025-00942-2

Figure Lengend Snippet: Altered cell cycle progression in T. brucei upon TbCDA-like overexpression. ( a ) Distribution of cells across different cell cycle phases after 2 days of overexpression induction, followed by propidium iodide staining and flow cytometry (FACS) analysis. Data are presented as mean ± SD from 3 independent experiments. ( b ) Quantification of nuclei (N) and kinetoplasts (K) after 2 days of overexpression induction, followed by DAPI staining and fluorescence microscopy. N* and K* denote elongated nuclei and kinetoplasts, respectively, indicative of mitosis or kinetoplast division. Data are presented as mean ± SD from approximately 300 cells from 3 independent experiments. Asterisks indicate significant differences determined by ANOVA. * p < 0.05; *** p < 0.001. The statistical significance analyse was performed by comparing the OE + DOX cell line with the Tb PF cell line. ( c ) Representative fluorescence microscopy images (Zeiss Axio Imager A1) after DAPI staining, illustrating the different cell populations.

Article Snippet: The statistical significance analyse was performed by comparing the OE + DOX cell line with the Tb PF cell line. ( c ) Representative fluorescence microscopy images (Zeiss Axio Imager A1) after DAPI staining, illustrating the different cell populations.

Techniques: Over Expression, Staining, Flow Cytometry, Fluorescence, Microscopy